Regulatory
PpsbD lead

Part:BBa_K3758005:Design

Designed by: Michael Burgis   Group: iGEM21_Marburg   (2021-09-12)


PpsbD, psbD promoter w/ synthetic leader


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 114
    Illegal XbaI site found at 108
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 114
    Illegal BamHI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 114
    Illegal XbaI site found at 108
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 114
    Illegal XbaI site found at 108
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

test


Source

Chloroplast of Nicotiana tabacum


References

Allison, L. A., & Maliga, P. (1995). Light-responsive and transcription-enhancing elements regulate the plastid psbD core promoter. The EMBO Journal, 14(15), 3721–3730. https://doi.org/10.1002/j.1460-2075.1995.tb00042.x

Occhialini, A., Piatek, A. A., Pfotenhauer, A. C., Frazier, T. P., Stewart, C. N., Jr., & Lenaghan, S. C. (2019). MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology. Plant Physiology, 179(3), 943–957. https://doi.org/10.1104/pp.18.01220